Fig. 2

Lentiviral particles expressing KIF3B shRNA in the PrL do not impact locomotor activity or produce anxiety. A Representative confocal images of the prelimbic cortices of C56Bl/6 mice injected with 200nL of sh-KIF3B-LV. Virus expressed for four weeks. Enlarged inset (right) shows neuronal-specific labeling. Scale 500 µm, 50 µm. B Experimental schema. C qRT-PCR analysis of RNA from tissue of the PrL after four weeks of virus expression of shKIF3B. Ct values were normalized to 18 s RNA for dCT and ddCT was calculated relative to the shScambled group levels. Student’s t test. *p < 0.05. Error bars are SEM. N = 4 per group. D–G Mice were injected with shScrambled-LV and shKIF3B-LV. After four weeks, mice underwent the open field test. Student’s t test. *p < 0.05. Error bars are SEM. N = 9 per group. F, G Measure of anxiety-like behavior during the first five minutes of the open field test. Data used for preparing plots are shown in Additional file 1: Table S2