Fig. 2
Cre/ROCK2fl/fl mice exhibit anxiety-like behaviors. a Cre/ROCK2fl/fl mice spent less time in the open arms of the elevated plus maze (EPM) compared to ROCK2fl/fl (t(50) = 3.140, **p = 0.0028). N = 28 ROCK2fl/fl mice (14 M, 14 F) and 24 Cre/ROCK2fl/fl (11 M, 13 F). b, c Light Dark Box Test. b Cre/ROCK2fl/fl mice spent less time in the light box compared to ROCK2fl/fl mice (t(49) = 2.557, *p = 0.0137) and c more time in the dark box (t(49) = 2.562, *p = 0.0135). N = 27 ROCK2fl/fl mice (13 M, 14 F) and 24 Cre/ROCK2fl/fl (11 M, 13 F). d, e Open field test for ROCK2fl/fl (N = 28: 14 males, 14 females) and Cre/ROCK2fl/fl (N = 25: 12 M, 13 F) mice. d Ambulatory counts and e ambulatory distance were measured. f Cre/ROCK2fl/fl mice did not show any changes in latency to enter the dark compartment in the passive avoidance test compared to ROCK2fl/fl mice. N = 14 ROCK2fl/fl mice (7 M, 7 F) and 12 Cre/ROCK2fl/fl (6 M, 6 F) mice at 8–9 months of age. Unpaired t-tests were used for all comparisons. Each point represents one mouse and error bars represent standard error of the mean. R2fl/fl, ROCK2fl/fl; Cre/R2fl/fl, Cre/ROCK2fl/fl