Fig. 6

Cre/ROCK2^fl/fl mice exhibit reduced apical spine head volume among dorsal CA1 pyramidal neurons. Dorsal CA1 (dCA1): a Spine volume of all dendrites combined was similar between groups. b There were no differences in the volume of thin, stubby, or mushroom spines between groups. c Cre/ROCK2^fl/fl mice show decreased apical spine volume (t(11) = 3.127, **p = 0.0096) compared to ROCK2^fl/fl mice. d There were decreased thin (t(11) = 2.348, *p = 0.0386), stubby (t(11) = 2.389, *p = 0.0359), and mushroom (t(11) = 2.627, *p = 0.0235) spine volume in Cre/ROCK2^fl/fl compared to ROCK2^fl/fl mice. e There was no difference in basal spine volume between groups. f There were no differences in the volume of basal thin and stubby spines between groups but, Cre/ROCK2^fl/fl mice had reduced mushroom spine volume (t(11) = 2.219, *p = 0.0485) compared to ROCK2^fl/fl mice. Ventral CA1 (vCA1): Cre/ROCK2^fl/fl mice had similar g overall spine volume and h spine volume of thin, stubby, or mushroom subclasses compared to ROCK2^fl/fl mice. There were no differences in overall i apical spine volume, j spine subclasses or k basal spine volume or volume of l basal spine subclasses. dCA1: N = 5 ROCK2^fl/fl mice (1 M, 4 F) and 8 Cre/ROCK2^fl/fl (5 M, 3 F) mice; vCA1: N = 5 ROCK2^fl/fl mice (2 M, 3 F) and 7 Cre/ROCK2^fl/fl (4 M, 3 F) mice at 8–9 months of age. Unpaired t-tests were used for all comparisons. Each point represents one mouse and the error bars indicate the standard error of the mean. R2^fl/fl, ROCK2^fl/fl; Cre/R2^fl/fl, Cre/ROCK2^fl/fl