Fig. 1
Effects of ketamine on the sEPSC of mPFC neurons. (A) Experimental scheme and the recording site in the mPFC. (B) Representative traces of sEPSCs. (C) WT mice displayed elevation in the frequency of the sEPSCs 1 h after ketamine (25 mg/kg) injection compared to their saline-injected counterparts, whereas GluN2D KO mice did not (n = 18-21 cells, 5-6 mice/group; ketamine effect, F1, 78 = 8.473, p = 0.0047; genotype, F1, 78 = 5.067, p = 0.0272; interaction, F1, 78 = 15.79, p = 0.0002; WT ketamine vs saline, p < 0.0001; GluN2D KO ketamine vs. saline, p = 0.7099). (D) No significant differences were observed in the amplitude of sEPSC. (E) Representative traces of paired-pulse ratio (PPR) at 150 ms inter stimulus interval (F, G) No significant differences were observed in PPR at any of the inter-stimulus intervals examined (50–200 ms) (n = 13-14 cells, 4-5 mice/group). Electrophysiology data are represented as mean ± SEM. sEPSC, PPR: 2-way ANOVA with Post hoc Sidak’s multiple-comparisons test. **** p < 0.0001. ns, nonsignificant