Fig. 6
From: The exit of axons and glial membrane from the developing Drosophila retina requires integrins
Knockdown of scb in eye-disc epithelium but not WG prevented the exit of R-cell axons and WG membrane. 3rd-instar larval eye–brain complexes were double-stained with MAb24B10 (green) and anti-Bdl (magenta). A–Aʺ Wild type. B–Bʺ when scb was knocked down in both eye-disc epithelium and WG by expressing UAS-scb-RNAi-JF02696 under control of ey3.5-GAL4 and Mz97-GAL4, most R-cell axons and WG membrane stalled in the eye disc. C–Cʺ In an individual in which scb was knocked down only in eye-disc epithelium by expressing UAS-scb-RNAi-JF02696 under control of ey3.5-GAL4, some R-cell axons and WG membrane exited the eye disc and stalled in the optic stalk. D–Dʺ WG-specific knockdown of scb by expressing UAS-scb-RNAi-JF02696 under control of Mz97-GAL4 did not affect R-cell axonal projection or WG membrane extension (n = 13). Scale bar: 20 µm