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Fig. 5 | Molecular Brain

Fig. 5

From: Long non-coding RNA lncC11orf54-1 modulates neuroinflammatory responses by activating NF-κB signaling during meningitic Escherichia coli infection

Fig. 5

Identification of IRAK1 as a binding protein of mgU2-30. A Silver staining of biotinylated mgU2-30-associated proteins in hBMECs. The mgU2-30-specific bands were highlighted with rectangle outlines. B Immunoblots of proteins from RAP assay by biotinylated mgU2-30 or negative control probe. C IRAK1 RIP followed by PCR assay of purified RNAs from hBMECs. The positive control U1 snRNA PCR product was also observed in the anti-SNRNP70 RIP. Densitometry was performed to analyze differences. “Null” columns represented the absence of densitometry data. Data were presented as mean ± SD from three independent assays. * p < 0.05, *** p < 0.001. D RNA FISH detecting mgU2-30 (red) combined with immunofluorescence staining of IRAK1 (green) in hBMECs. DAPI staining was shown in blue. Scale bars, 20 μm

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Molecular Brain

ISSN: 1756-6606