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Fig. 6 | Molecular Brain

Fig. 6

From: Long non-coding RNA lncC11orf54-1 modulates neuroinflammatory responses by activating NF-κB signaling during meningitic Escherichia coli infection

Fig. 6

mgU2-30 promotes IRAK1 oligomerization and auto-phosphorylation. A Immunoprecipitation of His-IRAK1 (or Flag-IRAK1) followed by immunoblotting (IB) analysis of Flag-IRAK1 (or His-IRAK1) in Flag‐tagged IRAK1 and His‐tagged IRAK1 pair co‐transfected hBMECs (Flag-tagged IRAK1/His-tagged empty vector, His-tagged IRAK1/Flag-tagged empty vector, or Flag-tagged empty vector/His-tagged empty vector pairs were also co-transfected as negative controls). B IB analysis of Flag-IRAK1 (or His-IRAK1) of hBMECs transfected with both tagged-IRAK1 constructs after His-IRAK1 (or Flag-IRAK1) immunoprecipitation to detect IRAK1 oligomerization in the presence or absence of mgU2-30. C and D Western blot analysis of IRAK1 and phosphorylated IRAK1 in hBMECs transfected with multiple dosages of lncC11orf54-1 (C) or mgU2-30 (D) (0, 100, 500, 1000, and 20,000 ng) under E. coli infection. β-actin was used as the loading control

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Molecular Brain

ISSN: 1756-6606