Fig. 1

Brain mapping of dopamine D1 and D2 receptor-positive neurons. a, b Whole-brain imaging of D1 and D2 receptor-positive neurons of D1-tdTomato and D2-tdTomato mice, respectively. Fluorescent histochemical observation of D1 receptor-expressing neurons (a) or D2 receptor-expressing neurons (b) using CUBIC. Images were acquired from horizontal (a-i, b-i) and sagittal (a-ii, b-ii) sections. Scale bars = 1000 μm. c The distribution of D1 receptor-expressing (c-i) and D2 receptor-expressing (c-ii) neurons in the NAcLat and NacMed of D1-tdTomato or D2-tdTomato mice, respectively. The slices were not immunostained and mounted directly with Dako fluorescent mounting medium. Scale bars = 100 μm