Fig. 3
From: The adaptor protein PICK1 targets the sorting receptor SorLA
The extreme C-terminus of SorLA recruits PICK1 but is dispensable for internalization. A COS7 cells were transfected with IL2R-SorLA-CD, IL2R-SorLA-CD-Del3, IL2R-SorLA-CD-VAA (green) and co-transfected with tdTomato-PICK1, tdTomato-Arfip1 or tdTomato-GGA2 (magenta) as indicated. Magnifications of selected areas (boxes) are shown as insets. IL2R-SorLA and tdTomato constructs were stained with anti-IL2Rα and anti-tdTomato respectively. B SY5Y cells were transfected with IL2R-SorLA-CD and IL2R-SorLA-CD-Del3. Cells were incubated with anti-IL2Rα at 4 °C, fixed after 0 or 30 min at 37 °C to allow internalization and immunostained. Three cells per time point and transfected construct are shown. Scale bars: 10 µm