Fig. 4

Conditional ablation of Hif1α in neuroepithelial cells causes neuronal apoptosis and consequently disturbs the formation of cortical layers in the cortex. Apoptotic cells and cortical neurons in coronal sections of WT and KO cortices at P0 are detected with anti-cleaved caspase3 and Tuj1 (anti-β-tubulin class III) (A, B), or anti-Satb2 and anti-Ctip2 (C, D) antibodies. Reelin expression in coronal sections of WT and KO cortices at E18.5 is detected with anti-Reelin antibody (E, F). Scale bars, 100 μm (A–F). WT, wild type; KO, knockout. Four (A–D) and three (E and F) independent experiments are performed and one representative image is shown, respectively