Fig. 3

High expression of Mbp in Tmem108 mutant mice. A Quantified relative mRNA expression of Mbp by qPCR in the whole brain (Male mice per group n = 3; Two-way ANOVA analysis, postnatal day P-value < 0.001, genotype P-value < 0.01, interaction P-value < 0.001, and Sidak's multiple comparisons test was conducted on indicated postnatal day). B, C Relative mRNA expression of Mbp by qPCR in gray matter, white matter (B) or corpus callosum (C) (Adult male mice, Tmem108 + / + mice n = 3, Tmem108 −/− mice n = 3; Unpaired T-test were made between the groups). D Representative image of MBP level in postnatal mice brain by western blotting (left panel); Quantification of MBP level in the western blotting (Tmem108 + / + mice n = 3, Tmem108 −/− mice n = 3; Two-way ANOVA analysis, postnatal day P-value = 0.0441, genotype P-value = 0.0122, interaction P-value = 0.0024, and Sidak’s multiple comparisons test was conducted at each postnatal day). E–F Representative image of MBP level in different areas of adult mice brain by western blotting (left panel); Quantification of MBP level in the western blotting (right panel), including gray matter, white matter (E), and CC (F) (T-test analysis, Tmem108 + / + mice n = 5, Tmem108 −/− mice n = 5; Two-way ANOVA and unpaired T-test analysis were used). G–I High MBP fluorescence intensity in the corpus callosum of Tmem108 mutant mice. G Representative images of MBP staining in mice CC. H MBP fluorescence area of CC in Tmem108 mutant mice (Tmem108 −/−) was not different from the control mice (Tmem108 + / +). I MBP fluorescence intensity of CC in Tmem108 mutant mice was higher than the control mice (Scale bar = 200 μm; Male mice per group, n = 3, unpaired T-test analysis). (Values are means ± SEM; n.s., not significant, *p < 0.05, **p < 0.01, **p < 0.001)