Fig. 6

Effects of LV-shRNA and LV-Rufy3 on the Rap1/Arap3/Rho/Fascin signaling axis after experimental SAH. a Representative bands of Fascin and Facin expressions. b, c Quantitative analysis of Fascin and Facin. The sham group was used as control. d Representative bands of ARAP3 and Rho expressions. e, f Quantitative analysis of ARAP3 and Rho. The sham group was used as control. g Double immunofluorescence of Fascin (green, Alexa Fluor 488) and β-tubulin III (NeuN; red, Alexa Fluor 555); nuclei were stained with DAPI (blue). Scale bars = 40 μm. h, i Quantitative fluorescent intensity analysis of Rufy3 and β-tubulin III expressions in different groups. The sham group was used as the standard. j Quantitative analysis of the length of neuronal axons in different groups. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Sham group; ^#P < 0.05, ^##P < 0.01 vs. LV-NC1 groups; ^&P < 0.05, ^&&P < 0.01, ^&&&P < 0.001 vs. LV-NC2 group; ^$P < 0.05 vs. LV-Rufy3 group