Fig. 1
From: New neurons in old brains: implications of age in the analysis of neurogenesis in post-mortem tissue
Doublecortin labeling is reduced with increased post-mortem interval. a Following post-mortem intervals of 0, 6, or 12 h, brains from 4- and 9-month-old rats (n = 4 were fixed for 48 h. The effects of age and post-mortem interval interacted significantly in influencing the b density of doublecortin+ cells (Two-Factor ANOVA, Age × Post-Mortem Interval Interaction; F2,18 = 5.448, p = 0.0141), c which we have also presented as a fold difference in doublecortin+ cell density relative to the mean density of the perfused groups of each age (Two-Factor ANOVA, Age × Post-Mortem Interval Interaction; F2,18 = 4.478, p < 0.0264). d This change in density of doublecortin+ cells occurred without any differences in dentate gyrus volume. e Age and post-mortem interval also interacted significantly to influence the optical density of doublecortin labelling in the granule cell layer (Two-Factor ANOVA, Age × Post-Mortem Interval Interaction; F2,18 = 12.34, p = 0.0004). Representative images of doublecortin+ cells in the subgranular zone in 4-month-old (f) and 9-month-old (g) rats with post-mortem intervals of 0, 6, and 12 h. Representative images of the morphology of doublecortin labelling in 4-month-old (h) and 9-month-old (i) rats with post-mortem intervals of 0, 6, and 12 h. Data are mean ± SEM. Post-hoc analyses of significant interactions in Two-Factor ANOVA used Newman Keuls. *p < 0.05