Fig. 4
From: Paraventricular hypothalamic vasopressin neurons induce self-grooming in mice
Chemogenetic inhibition of PVHAVP neurons reduces self-grooming in freely moving mice. A Schematic representation of viral vector AAV-EF1α-DIO-hM4Di-mCherry injection in the PVH of Avp-Cre mice. B A representative coronal brain section containing the PVH prepared from an Avp-Cre mouse with bilateral focal injections of AAV-EF1α-DIO-hM4Di-mCherry in the PVH. 3v, third ventricle; Scale bar, 300 μm. C Time courses of behaviors of 2 mice expressing hM4Di in PVHAVP neurons following saline or CNO administration. D Time spent in self-grooming, E number of grooming bouts, and F mean duration of grooming bouts for 60 min following saline or CNO administration. Values are mean ± SEM; n = 6; *p < 0.05, **p < 0.005 by paired t-test