Fig. 2

DS-specific CDK5-KD affects the motor activity of mice. A The locomotor activity test was performed 14 days after CDK5 or NC-sgRNA microinjection. Movement was recorded for 60 min and reported as distance in centimeters (cm) per 10 min. B, C ANOVA analysis of total distance traveled (B) and averaged speed (C) in the locomotor test of WT, DS-CDK5-NC, and DS-CDK5-KD mice. D Activity heatmap of mice in the locomotor test. Blue indicates low activity, and red indicates high activity. E Statistical analysis of activity duration (%) in the locomotor test chamber. F WT and DS-CDK5-NC mice exhibited normal activity with free-walking, as in the left and middle panels, whereas DS-CDK5-KD mice spent more time immobile and curled up, as in the right panel, during the test period. G Quantitative analysis of the curling up behavior in the locomotor test chamber. Data are represented as the mean ± SEM, n = 20 mice per group. One-way ANOVA, Tukey’s multiple comparisons test, *p < 0.05, **p < 0.01, ***p < 0.001 vs WT