Fig. 6

CDK5 knockdown perturbs synaptic transmission in the DS. A Schematic depiction of electrode placements and action potential properties recorded from MSNs in the DS. B Immunohistochemical identification of recorded neurons is GAD-expressing MSNs in DS slices. Scale bar: 10 μm. C Representative mEPSC traces recorded from MSNs from WT, DS-CDK5-NC, and DS-CDK5-KD mice. Scale bars = 20 pA/1 s. D Cumulative histograms and quantification of mEPSC frequency and amplitude from (C). E Representative sIPSC traces recorded from MSNs of WT, DS-CDK5-NC, and DS-CDK5-KD mice. Scale bars = 20 pA/1 s. F Cumulative histograms and quantification of mEPSC frequency and amplitude from (E). Data are represented as the mean ± SEM, n = 12 MSNs from four mice per group. One-way ANOVA, Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001 vs WT