Fig. 2
From: Genome-wide translation control analysis of developing human neurons
Translational Control analysis of genes expressed during human neuronal differentiation. A Ribosome Profiling versus RNA sequencing expression plots at selected differentiation stages. Blue: High translation efficiency genes (TE > 3); Red: low translation efficiency genes (TE < 0.33), FDR < 0.01. B RNAseq versus Riboseq fold-change plots comparing ED and Neurons with NPC. Genes were classified according to their regulation with the parameters indicated criteria. Blue represents Up-regulated genes, with no change in translation efficiency; Red indicates Down-regulated genes, with no change in translation efficiency; Dark blue indicates genes with increased translation efficiency and significative increase of total riboseq counts between cells; Dark red indicates genes with decreased translation efficiency and significative decrease of total riboseq counts between cells; Black indicates genes with increased translation efficiency without a significative increase in total riboseq counts between cells; Green indicates genes with decreased translation efficiency without significative decrease of total riboseq counts between cells. Gray indicates genes without regulation. C) Subcellular localization analysis of translation-regulated transcripts. Genes from each regulatory category in B were compared with human axonal transcriptome [25], synaptic bouton proteome [26] and monosome/polysome enriched transcripts in soma/neuronal neuropil [79]. Data is presented as Fisher exact test p-values (X-axis) to the percentual intersected relative to total genes in the subcellular localization category list (Y-axis). D IPA GO enrichment analysis of TE regulated genes in Neurons (TE FC > 2, FDR < 0.01)