Fig. 2
From: Increased spine PIP3 is sequestered from dendritic shafts
PIP3 is increased in dendritic spines but not dendritic shafts in response to high potassium-induced depolarization. A Fluorescence lifetime imaging of FLIMPA3. A color gradient was used to represent PIP3 levels, with a reddish color indicating a shorter fluorescence lifetime and higher PIP3 levels. White arrows indicate dendritic spines. The fluorescence lifetime values of dendritic shafts corresponding to dendritic spines were analyzed from the regions indicated by white rectangles in the image before KCl stimulation. White bar = 1 µm. B Time-course analysis of the fluorescence lifetime of dendritic spines and shafts. Asterisks denote a statistically significant difference in the fluorescence lifetime difference between dendritic spines and shafts (p < 0.05)