Fig. 5

Effects of linopirdine and retigabine on AP firing patterns at nodes of Ranvier. A, B Sample traces show APs evoked at a NR by current steps in the absence (control, A) and presence of 100 µM linopirdine (B). The grey and black traces in (A) and (B) show APs evoked with rheobase (grey) and 3 × rheobase (black) stimulation, respectively. Recordings were performed under the whole-cell current-clamp configuration and step currents were injected into the NR through recording electrodes. C Left panel, percent of NRs with single AP firing (open bar) and multiple AP firing (black bar) before (control, n = 12) and following the application of linopirdine (n = 12). Right panel, Numbers of APs before (control, n = 12) and following the application of linopirdine (n = 12). D Similar to C except retigabine was tested (n = 7). Data represent mean ± SEM, ns, not significantly different, *p < 0.05, ***p < 0.001, paired Student’s t-Test