Fig. 3
From: Knockdown of myorg leads to brain calcification in zebrafish
myorg cDNA rescued the E2I2-MO induced calcification phenotype. A Dorsal views of zebrafish embryos injected with control morpholino oligonucleotides (Control-MO), myorg-E2I2-MO, and E2I2-MO combined with myorg cDNA. These embryos were stained with calcein at 3 dpf. Compared to Control-MO (a–c), myorg-E2I2-MO showed potent brain calcifications as green fluorescence in the zebrafish brain (d–f), E2I2-MO plus myorg cDNA resulted in reduced calcification nodules (g–i). a, d, g bright field; b, e, h calcein staining; the merged images are shown in c, f, i. B Quantification of the calcification number in the brain in myorg-E2I2-MO with or without myorg cDNA injection to zebrafish at 3 dpf compared to that in Control-MO injected zebrafish. Means ± SEM, n = 10, Student’s t-test, ****P < 0.0001. Scale bar, 50 μm