Fig. 1

Rapid enhancement of transgene expressions in primary cultured cortical neurons by AAV2-retro YF mutation. A A schematic of the YF mutation sites of AAV2-retroYF. Note that the Y730F in AAV2YF shifts Y740F due to the 10 amino acids of the retro sequence insertion. B A schematic view of the experimental procedure of in vitro AAV assay on the primary cultured neuron. C Representative images of EGFP (green) and MAP2 (magenta) in AAV2-retro-CAG-EGFP (top) vs AAV2-retroYF-CAG-EGFP (bottom) applied cell cultures at 1 week post infection. The white arrows indicate EGFP- and MAP2-double positive neurons. D The histogram of the EGFP fluorescent intensity of the double positive neurons (n = 155 [original], n = 385 [mutant], p < 0.001, U = 18,296, Wilcoxon's rank sum test). E Representative images of EGFP (green) and MAP2 (magenta) in AAV2-retro-CAG-EGFP (top) vs AAV2-retroYF-CAG-EGFP (bottom) applied cell cultures at 2 week post infection. The white arrows indicate EGFP and MAP2 double positive neurons. F The histogram of the EGFP fluorescent intensity of double positive neurons at 2 week post infection (n = 172 [original], n = 383 [mutant], p = 0.11, U = 30,146, Wilcoxon's rank sum test). All scale bar, 50 μm