Fig. 2
From: Silencing of hypothalamic FGF11 prevents diet-induced obesity
ARC Fgf11 knockdown increases heat generation, VO2, VCO2, and BAT thermogenesis without changing locomotor activity. Adult male C57BL/6 mice fed HFD for 2 weeks after bilateral injection of shFgf11-expressing lentivirus into the ARC. A–F Heat generation, VO2, VCO2, RER, total activity at z-axis (ZTOT), and ambulatory activity at x-axis (XAMB). G–H Infra-red images of BAT temperature and max BAT temperature on day 14 post-surgery. I mRNA expression of 4 representative thermogenic markers on day 14 after ARC Fgf11 knockdown: Ucp1, Pgc1α, Dio2, Prdm16. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 (non-silencing shRNA control versus shFgf11). n = 5–10 mice/group