Fig. 1
From: Proteins secreted by brain arteriolar smooth muscle cells are instructive for neural development
HBVSMC-CM promotes neuron development by accelerating single neurite maturation and increasing neurite numbers. a–c Representative images of hippocampal neurons stained with an anti-Tuj1 antibody (green) and counterstained with Hoechst (blue). Specialized neurites are outlined with white dashed lines, and non-specialized neurites with large growth cones are outlined with magenta dashed lines. d Quantification of neurons in which all neurites were specialized in the control and HBVSMC-CM groups. e, f Cumulative curve of the percentages of neurons with different neurite numbers (e) and neurite lengths (f) at 24, 48, and 72 h. g–i Quantification of average total neurite length per neuron at 24 (g), 48 (h), 72 h (i). j, k Sholl-analysis of 48-h-old (j) and 72-h-old (k) hippocampal neurons with or without HBVSMC-CM treatment