Fig. 4

Increased neuronal activity of connectivity subnetworks after CSD. Automated analysis of c-fos positive cell density in mouse brains harvested 3 h after CSD (n = 5) or EYFP group (n = 5). The raw data and c-fos density heat map were 25-um coronal section. A Active areas in orofaciopharyngeal subnetwork included primary somatosensory area nose layer 5 and 6, visceral area layer 6 and gustatory areas layer 4 on the ipsilateral hemisphere, and primary somatosensory area nose layer 6, visceral area layer 2/3 and 4 and gustatory areas layer 4 on the contralateral hemisphere. B The activation regions in upper limb subnetwork was primary somatosensory area upper limb layer 2/3 on the contralateral hemisphere. The contralateral primary somatosensory area lower limb and trunk layer 1 and 2/3 of lower limb/trunk subnetwork were activated. C Active areas in whisker subnetwork included ipsilateral primary somatosensory area barrel field and contralateral primary somatosensory area barrel field layer 4,5 and 6. D The activation regions in first medial subnetwork included ipsilateral anterior cingulate area dorsal parts (dACA) and contralateral anterior cingulate area dorsal part layer 6. E The activation regions in first medial subnetwork included ipsilateral retrosplenial area dorsal part (dRSP) layer 6 and contralateral RSP lateral agranular part layer 2/3 and 5. F Active areas in temporal subnetwork of the lateral subnetwork were temporal association areas (TEa), perirhinal area layer 6 and entorhinal area lateral part layer 6 on the ipsilateral hemisphere. G The activated regions in ventromedial prefrontal cortex (vmPFC) included ipsilateral orbital area medial part (mORB), orbital area ventrolateral part (vlORB), prelimbic area (PrL) layer 1, 2/3 and 5, infralimbic area (ILA), agranular insular area dorsal part (AId) layer 2/3 and contralateral mORB layer 2/3 and 5 and orbital area lateral part (lORB) layer 6