Fig. 4

Decreased expression of the phosphorylated leucine-rich repeat kinase 2, but not total leucine-rich repeat kinase 2 expression in the 14-3-3γ heterozygous mice brain. a Schematic diagram of the domain structure of leucine-rich repeat kinase 2 (LRRK2) b Representative western blotting images of the pLRRK2 at S910, at S935, and LRRK2 expression in the whole brain, the striatum (ST), and substantia nigra (SN) homogenates of the 14-3-3γ HET mice and CTL mice. c–k Quantitative graphs of pLRRK S910 (c–e), S935 (f–h), and LRRK2 (i–k) expression in the whole brain, the ST, and SN homogenates of the 14-3-3γ HET mice and CTL mice (n = 3 per group). Results are presented as means ± SEM; *P < 0.05, **P < 0.01 and ***P < 0.001. (CTL littermate wild-type control, HET heterozygous, P-P I protein–protein interaction, pLRRK phosphorylated leucine-rich repeat kinase 2, LRRK2 leucine-rich repeat kinase 2, ST striatum, SN substantia nigra, SEM standard error of mean, ns non-significant)