Fig. 6

BDNF rescued the synaptic tagging in middle-aged mice. BDNF was applied in the brain slices of middle-aged male mice. Tagging LTP recorded around site S2 from the brain slices of the BDNF applied /unapplied in middle-aged male mice (a). LTP was recorded around site S1 from the brain slices of the same mice (b). Trk B, CREB levels were detected by western blot in three groups, adult mice, middle-aged mice, and BDNF-applied middle-aged mice (c). The intensity level of Trk B and CREB in each group, n = 5 for each group (d). The Markov Random Field model was introduced to smooth and visualize the low-resolution 8 × 8 MED64 data. The temporal evolving of the 3D surface on site S2 on the brain slice of the control middle-aged male mouse (e). The temporal evolving of the 3D surface on site S2 on the brain slice of the BDNF-incubated middle-aged male mouse (f)