Fig. 1

Sexual dimorphic Calcr expression and gene profiles of Calcr + cells in the medial amygdala. A Representative coronal brain sections of both sexes, showing the distribution of Calcr immunoreactivity (Calcr-ir, black) and NeuN counterstaining (red) in subregions of the amygdala in female or male mice. MeAad anteroventral part of the medial amygdala, MeApd posterodorsal part of the medial amygdala, MeApv posteroventral part of the medial amygdala, CeM medial part of the central amygdala, CeL lateral part of the central amygdala, CeC capsular part of the central amygdala, AA anterior amygdaloid area, LA lateral amygdala, BLA basolateral amygdala, BMA basomedial amygdala. Scale bars, 500 μm. B Number of Calcr-ir neurons in each subregion of female or male mice (n = 4 mice per group). C Sections stained with Gad67 mRNA (blue) by ISH together with anti-Calcr (brown) antibody. Arrowheads indicate double-labeled cells. Scale bars, 500 μm (left) and 50 μm (right). D Sections stained with anti-ERα (black) by IHC together with anti-Calcr (brown) antibody. Scale bars, 500 μm (left) and 50 μm (right). E Sections stained with anti-amylin (black) by IHC together with anti-NeuN (red) antibody. Scale bars, 500 μm (left) and 50 μm (right). Asterisks in (B) denote significant differences between two groups (Welch’s unpaired t-test, *P < 0.05). Graphs represent mean ± SEM. See Additional file 1 for detailed statics