Fig. 1
From: Tyrosine phosphatase STEP61 in human dementia and in animal models with amyloid and tau pathology
Modulation of STEP61 in human dementias and representative mouse models. A Immunoblot of RIPA-extracted tissue from the superior frontal cortex of patients diagnosed with AD, MCI and healthy controls and ordered by Braak stages. Of note, the controls in panels A and C are the same samples, only that in A they are grouped according to their Braak stage (with most controls being clinically normal displaying advanced Braak stages upon autopsy), whereas in C all controls are combined given that for FTD-tau (PiD, CBD and PSP) the Braak staging method is not available. Protein levels were normalised to actin. One way ANOVA multiple comparisons correction was performed on each data set; *p < 0.05; active STEP p = 0.0031, total STEP p = 0.0036, PSD-95, p = 0.0027, tau, p = 0.5730. B Immunoblot and quantification of extrasynaptically enriched fractions from 6-month-old APP23 mice (n = 9) and wild-type (WT) littermate controls (n = 9). Quantification of active and total STEP61 normalised to GAPDH; unpaired t-test, active STEP p > 0.5, and total STEP p = 0.0315. C Immunoblot of RIPA-extracted tissue from the superior frontal cortex of FTD-tau (n = 8) ordered by disease and PSP severity (Braak staging) and controls (n = 8). AT8-positive tau was not detectable in FTD-tau patients. Protein levels were normalised for β-actin. One way ANOVA with multiple comparisons correction performed on each data set; active STEP p = 0.0299, total STEP p = 0.0213, PSD-95, p = 0.0043, tau p = 0.0708. D Immunoblot of the RIPA fraction of cortical tissue from 2-month-old K3 mice (n = 8) and WT littermate controls (n = 8). Quantification of total and active STEP61 normalised to GAPDH; unpaired t-test, active STEP p = 0.1764, total STEP p = 0.0523. E Immunoblot of the RIPA fraction of cortical tissue from 5-month-old K3 mice (n = 5) and WT littermates (n = 5) probed for total and active STEP61. Quantification of active and total STEP61 normalised to GAPDH; unpaired t-test, active STEP p = 0.0007, total STEP p = 0.0015 (see Additional file 2: Statistical information)