Fig. 6
Impaired proliferation of neural progenitor cells in Ggps1 cKO mice. A, B Representative fluorescence images for co-staining of Pax6/BrdU. Cerebellar sections at E17.5 (A) and P0 (B) were used. The immuno-reactivity of Pax6 + /BrdU + cells was qualitatively decreased in Ggps1 cKO mice at P0. The scale bars were indicated in the images. C The average number of Pax6 + /BrdU + cells in the EGL. There was significant difference between control and Ggps1 cKO mice at E17.5 and P0 (*P = 0.018, ***P = 3.5 × 10–6, respectively; n = 6 mice per group at E17.5, n = 5 mice per group at P0). D, E Representative fluorescence images for co-staining of Pax6/Ki67. Mice at E17.5 (D) and P0 (E) were used. The immuno-reactivity of Pax6 + /Ki67 + cells was decreased in Ggps1 cKO mice. The scale bars were indicated in the images. F The average number of Pax6 + /Ki67 + cells in the EGL. There was significant difference between control and Ggps1 cKO mice at E17.5 and P0 (*P = 0.011, ***P = 2.3 × 10–4, respectively; n = 5 mice per group). G Representative images for cultured neurospheres. Cerebellar tissues from control and Ggps1 cKO mice were used for neurosphere cultures. The scale bar is 100 μm. H The average diameter of neurospheres at 7 days in vitro. There was significant reduction in the Ggps1 cKO group compared with the control (***P = 9.9 × 10–33; n = 28 neurospheres cultured from 9 WT mice, n = 36 neurospheres cultured from 9 cKO mice)