Fig. 5

Maged1 deficiency suppresses cell apoptosis in MPP⁺-intoxicated cells and MPTP-treated mice. A SH-SY5Y cells were transfected with NC or si-Maged1; after 36 h, cells were treated with 200 μM MPP⁺ for 48 h and cell viability quantified using the CCK8 assay. B SH-SY5Y cells were transfected with Vector or Maged1-HA; after 24 h, cells were treated with 200 μM MPP⁺ for 48 h and cell viability was quantified using the CCK8 assay. C SH-SY5Y cells transfected with NC or si-Maged1 were treated with 200 μM MPP⁺ for 48 h, apoptotic cells were stained using PI, measured using flow cytometry, and quantified. D SH-SY5Y cells were transfected with NC or si-Maged1 and treated with 200 μM MPP⁺ for 48 h and cell lysates were detected for Bcl-2, Bax, Pro-caspase-3, and Cleaved PARP; GAPDH was used as a loading control. E–H Quantification of Bcl2/GAPDH (E), Bax/GAPDH (F), Pro-caspase-3/GAPDH (G), and Cleaved PARP/GAPDH (H) from results of (D). I Original bands of Bcl2 and Bax from midbrain extracts; actin was used as a loading control. J, K Quantification of Bcl2/Actin (J) and Bax/Actin (K) from the results of (I), n = 4 for each group. For A–H, results are representative of three separate experiments. Data are shown as means ± SE. *P < 0.05, **P < 0.01, ***P < 0.001, ^#P < 0.05, ^##P < 0.01, ^###P < 0.001. NC normal control, KD knock down