Fig. 1

Effects of oIAPP on HBVP in the microvascular model. A shows capillary-like structures (CLS) formed by HBVP and endothelial cells co-cultured on a gel matrix. Higher magnification of (A) is seen in (B). Scale bars in (A) and (B): 200 µm. In (C) and (D) CLS after treatment with vehicle (C) and oIAPP (D), with higher proportion of round-shaped HBVP (black arrows) in the latter. Scale bar in (C) and (D): 100 µm. Quantification of the proportion of round-shaped HBVP per CLS after stimulation is seen in (E). Images in (F) and (G) show CLS stained with green Cell Tracker (F) and trypan blue (G), where the red arrow indicates a round HBVP negative for trypan blue and the blue arrow indicates a trypan blue-positive round HBVP. Scale bar in (F) and (G): 50 µm. The quantification of the proportion of trypan blue-positive HBVP among total round HBVP per CLS after stimulation is shown in (H). Images (A) and (B) were acquired with the 4 × objective, images in (C), (D), (F) and (G) were acquired with the 10 × objective. Experiments in (E) and (H) were performed in 6 replicates and data was analyzed using student t-test. Each point represent the mean ± SD. Significant difference at **p < 0.01