Fig. 1

Preference for umami or bitter in the two-bottle test in prolonged taste exposure mice. A Experimental paradigm of prolonged taste exposure and two-bottle test. B Intake of water and umami during 15-min two-bottle test. C Preference ratios of umami. Preference ratios were calculated as the ratio of the umami intake to the total intake. D Access duration to water or umami bottle. E Access ratio of umami bottle. F Intake of water and bitter during 15-min two-bottle test. G Preference ratios of bitter. H Access duration to bitter bottle. I Access ratio of water or bitter bottle. Each circle represents results from one mouse. Data are represented as mean ± SEM. Water group, n = 8; Umami group, n = 9; Bitter group, n = 7. *p < 0.05, ***p < 0.001 (paired t-test); ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, ^####p < 0.0001 (Tukey’s post hoc test); ^†p < 0.05, ^††††p < 0.0001 (one sample t-test)