Fig. 3

SOX2OT knockdown suppresses apoptosis. a–c Fluorescent images showing virus-transfected HT22 cells and the stable knockdown of SOX2OT; SOX2OT expression levels detected using RT-PCR in the Con and LPS groups. *P < 0.05, ****P < 0.0001 vs. Lv-neg; ^####P < 0.0001 vs. Lv-neg + LPS. d HT22 Lv-neg or Lv-SOX2OT cells were treated with LPS (100 ng/mL) for 24, 48, and 72 h, and then cell viability was determined. *P < 0.05 vs. Lv-neg; ^#P < 0.05 vs. Lv-neg + LPS. e Activity of lactate dehydrogenase in HT22 Lv-neg or Lv-SOX2OT cells cultured with LPS (100 ng/mL) for 24, 48, and 72 h using the LDH kit. *P < 0.05, ***P < 0.0003 vs. Lv-neg;^####P < 0.0001 vs. Lv-neg + LPS. f Apoptosis detected using flow cytometry. ****P < 0.0001 vs. LV-neg; ^####P < 0.0001 vs. Lv-SOX2OT + LPS; ^&&&&P < 0.0001 vs. Lv-neg. g Western blot analysis showing Bax and Bcl-2 protein expression after transfection with Lv-SOX2OT or Lv-neg lentiviral vectors. ****P < 0.0001 vs. Lv-neg; ^####P < 0.0001 vs. Lv-SOX2OT + LPS; ^&&&&P < 0.0001 vs. Lv-neg; n = 4