Ankyrin2 is essential for neuronal morphogenesis and long-term courtship memory in Drosophila

Table 1 Frequency of mushroom body phenotypes resulting from knock down of Ank2

Genotype	elav > w(CS10)	elav > Ank2 RNAi1	elav > Ank2 RNAi1	elav > Ank2 RNAi1	OK107 > w(CS10)	OK107 > Ank2 RNAi2
Temperature	25 °C	22 °C	25 °C	27 °C	25 °C	25 °C
n	40	30	30	16	40	46
Thin α/β or γ lobes	0%	37%	23%	62%	0%	4%
Fused β lobes	0%	13%	10%	0%	0%	0%
Outgrowth/guidance defect	0%	37%	23%	50%	0%	7%
Absent α/β or γ lobes	0%	6%	40%	50%	0%	13%
Total % of brains with defects	0%	73%	73%	100%	0%	35%

As the phenotypes often differ across the two hemispheres of one brain (such as observed in Fig. 2B,D), the percentage of brain hemispheres displaying each phenotype was calculated from the total number of brain hemispheres analyzed for each genotype (n) at 22, 25 and 27 °C. The total percentage of brains displaying defects was also calculated for each genotype. Expression of Ank2 RNAi1 resulted in a significant number of brains with defects at all temperatures (p < 0.001), and there was an increased number of brains with defects when raised at higher temperatures (27 °C compared to 22 °C, Fisher’s Exact Test p < 0.001). Note that some brain hemispheres displayed thin lobes as well as outgrowth and/or guidance defects therefore the sum of percentage defects may be higher than 100%. Expression of Ank2 RNAi2 also resulted in significantly more brains with defects than controls at 25 °C (p = 0.0042).

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