Fig. 1

Δ⁹-THC produces spinal analgesia in mice that is Cav3.2 channel-dependent. Dose response action of Δ⁹-THC (delivered 20 min before formalin) in the a first and b second phases of the formalin test. Each bar represents the mean of 5 animals, error bars denote S.E.M. Data are representative of 2 independent sets of experiments. Statistical analyses were performed by two-way ANOVA followed by Tukey's test. Asterisks denote a significant difference, **P < 0.01 and ***P < 0.001 when compared with the control groups. c Mechanical threshold of PSNI mice 45 min after treatment with Δ⁹-THC (10 ug/i.t.). Bars represent the mean of 7 animals, error bars denote S.E.M. Data are representative of 2 independent sets of experiments. Two-way ANOVA followed by Tukey's test revealed significance, ###P < 0.001 and ****P < 0.0001 when compared with the control groups. d Time-course of the effect of Δ⁹-THC (10 μg/i.t.) on thermal withdrawal latencies of CFA-injected male mice. e Effect on CFA-treated female mice when evaluated 45 min following treatment. In d and e, error bars are S.E.M. Data are representative of 2 independent sets of experiments Two-way ANOVA followed by Tukey's test revealed statistical differences, *P < 0.05 **P < 0.01 or ***P < 0.001 when the CFA + treated group is compared with the CFA + vehicle control group, and ^###P < 0.001 when the PBS group is compared with the  control groups. f, g Comparison of the effect of 10 μg/i.t. Δ⁹-THC on CFA-injected wild type and f CB₁, g CB₂, and h Cav3.2 knockout mice. Each bar represents the mean of 6–10 mice, error bars are S.E.M. Data are representative of 2 independent sets of experiments. Two-way ANOVA followed by Tukey's test revealed statistical differences, *P < 0.05 or ****P < 0.0001 when compared with the control group