Fig. 4

Bcat1 regulates the proliferation of the NSCs. A, B The percentage of Bcat1⁺GFP⁺ cells are decreased in Bcat1 knockdown brains. The mouse was electroporated at E13.5 and killed at E18.5. The bar graph shows the percentage of Bcat1⁺GFP⁺ cells relative to the control Bcat1⁺GFP⁺ cells (n = 3 independent experiments; ***P < 0.001; bars represent mean ± S.E.M). The scale bar represents 50 μm. C, D The GFP⁺ cells are decreased in Bcat1 knockdown brains. The mouse brain was electroporated at E13.5 and BrdU (50 mg/kg) was injected 24 h prior to killing at E15.5. (n = 3 independent experiments; **P < 0.01; bars represent mean ± S.E.M). The scale bar represents 50 μm. E–G The percentage of GFP⁺ BrdU⁺ cells and GFP⁺ Ki67⁺ cells are decreased in Bcat1 knockdown brains. The mouse brain was electroporated at E13.5 and BrdU (50 mg/kg) was injected 24 h prior to killing at E15.5. The bar graph shows the percentage of GFP⁺ BrdU⁺ cells and GFP⁺ Ki67⁺ cells relative to the total GFP-positive cells (n = 3 independent experiments; **P < 0.01; bars represent mean ± S.E.M). The scale bar represents 20 μm