Fig. 5

Bcat1 Knockdown causes the abnormal neuronal development. A–C The percentage of GFP⁺ Satb2⁺ cells and GFP⁺Ctip2⁺ cells are decreased in Bcat1 knockdown brains. The mouse was electroporated at E13.5 and killed at E16.5. The bar graph shows the percentage of GFP⁺ Satb2⁺ cells and GFP⁺ Ctip2⁺ cells relative to the control GFP⁺ Satb2⁺ cells and GFP⁺ Ctip2⁺ cells (n = 3 independent experiments; **P < 0.01; bars represent mean ± S.E.M). The scale bar represents 20 μm. D, E The GFP + cells are decreased in Bcat1 knockdown brains. The mouse brain was electroporated at E13.5 and killing at E16.5. (n = 3 independent experiments; **P < 0.01; bars represent mean ± S.E.M). The scale bar represents 50 μm. F–H The percentage of GFP⁺ Satb2⁺ cells and GFP⁺ Ctip2⁺ cells are decreased in Bcat1 knockdown brains. The mouse brain was electroporated at E13.5 and killing at E18.5. The bar graph shows the percentage of GFP⁺ Satb2⁺ cells and GFP⁺ Ctip2⁺ cells relative to the control GFP⁺ Satb2⁺ cells and GFP⁺ Ctip2⁺ cells (n = 3 independent experiments; **P < 0.01; bars represent mean ± S.E.M). The scale bar represents 20 μm. I, J The GFP⁺ cells are decreased in Bcat1 knockdown brains. The mouse brain was electroporated at E13.5 and killing at E18.5. (n = 3 independent experiments; **P < 0.01; bars represent mean ± S.E.M). The scale bar represents 50 μm