Fig. 2

Effect of FTY720 on microglial activation in short-term cuprizone-exposed mice. A–C HIP. A Representative image of Iba1 immunostaining in the HIP. Blue: DAPI nuclear stain, Green: Alexa488 (Iba1). B Fluorescence intensity measurement of Iba1 signal in the HIP (n = 3 per group). C mRNA expression levels of Iba1 in the HIP. βActin was used for internal control, and samples were normalized to Cont-Veh. (n = 6 per group). D, E CC. D Fluorescence intensity measurement of Iba1 signal in the CC (n = 3 per group). E mRNA expression levels of Iba1 in the CC. F, G STR. F Fluorescence intensity measurement of Iba1 signal in the STR (n = 3 per group). G mRNA expression levels of Iba1 in the STR. βActin was used for internal control, and samples were normalized to Cont-Veh. (n = 6 per group). H, I Three-dimensional morphology analysis. H Representative image of z stack imaging. I Microglial ramification ratio. B–G Mean ± SEM. Tukey test *p<0.05, **p < 0.01. 2way ANOVA ^#p < 0.05, ^##p < 0.01, ^####p < 0.0001. I Violin Plot Min-Max values. Dotted lines indicate first, second, and third quartiles. 2way ANOVA ^####p < 0.0001