Fig. 1
CF selective gene delivery using Htr5B-tTA mice and AAV
A Experimental scheme. AAVs encoding ChR2-YFP with or without C1ql1 were injected into the ION. The right panel shows YFP signals in the ION. Scale bar, 500 μm. B Expression levels of C1ql1 in the ION. Immunohistochemical staining shows total C1ql1, exogenous HA-C1ql1 and YFP in the ION infected with AAV-CTRL and AAV-C1ql1. DAPI staining shows the nucleus. Scale bar, 20 μm. C Quantification of C1ql1 immunoreactivity in the soma of IONs (B2). p = 0.0389, Two-tailed Welch’s t-test; n = 4 mice each. D Selective expression of YFP and C1ql1 in the CFs. No YFP signals were detected in the mossy fibers in the granular layer. ML, molecular layer; PCL, Purkinje cell layer; GL, granular layer. Scale bar, 40 μm. E Immunohistochemical staining of vGluT2 (magenta), YFP or HA-C1ql1 (green) and calbindin (blue) indicates accumulation of HA-C1ql1 at CF synapses. Scale bar, 10 μm. Bars represent mean ± SEM. *p < 0.05