Fig. 2
Increased C1ql1 levels in CFs allow adult PCs to be innervated by multiple CFs
A Experimental scheme for recording CF-EPSCs. B Representative CF-EPSC traces from wild-type PCs. The blue bar indicates the timing of light stimulation. Increasing the light intensity elicited a single EPSC in control slices (B1) in an all-or-none manner, but multiple EPSCs with a slower rise time in slices overexpressing C1ql1 (B2). C Total CF-EPSC amplitude. The graph shows the sum of the peak amplitudes of single CF-EPSCs or multiple CF-EPSCs. p = 0.0372, two-tailed Welch’s t-test; n = 28 cells from 4 mice (CTRL); n = 29 cells from 5 mice (+ C1ql1). D The percentage of the number of CFs innervating single PCs. The number of EPSCs evoked by distinct CF activation thresholds (number of steps) is shown. p = 0.0234, Mann–Whitney U test; n = 51 cells from 4 mice (CTRL); n = 49 cells from 5 mice (+ C1ql1). E Average of the 10–90% rise time of CF-EPSCs. p = 0.0028, two-tailed Welch’s t-test; n = 28 responses from 4 mice (CTRL); n = 46 responses from 5 mice (+ C1ql1). F Representative CF-EPSC traces from Bai3 knockout PCs (left: CTRL, right: +C1ql1). G Total CF-EPSC amplitude. The graph shows the sum of peak amplitudes of single CF-EPSCs or multiple CF-EPSCs. p = 0.8534, two-tailed Welch’s t-test; n = 31 cells from 3 mice (CTRL); n = 42 cells from 4 mice (+ C1ql1). H The percentage of the number of CFs innervating single PCs. p = 0.6159, Mann–Whitney U test; n = 38 cells from 3 mice (CTRL); n = 50 cells from 4 mice (+ C1ql1). I Average of the 10–90% rise time of CF-EPSCs. p = 0.0609, two-tailed Welch’s t-test; n = 33 responses from 3 mice (CTRL); n = 46 responses from 4 mice (+ C1ql1). Bars represent mean ± SEM. **p < 0.01; *p < 0.05; ns, not significant