Fig. 4
From: Specific vulnerability of iPSC-derived motor neurons with TDP-43 gene mutation to oxidative stress
Evaluation of stress granule formation induced by oxidative stress. A Conditions of hydrogen peroxide administration and timing of G3BP1 expression evaluation. B, D Immunostaining of stressed neurons for the stress granule marker G3BP1. C, E Quantitative analysis of the percentage of stressed neurons. The percentage of stressed motor neurons was higher in the A382T group than in the control group. There were no significant differences in the percentage of stressed sensory neurons. Scale bars, 20 μm in B, D. C, E n = 5 in each of the three motor neuron groups. n = 3 in each of the three sensory neuron groups. The data are presented as the mean ± SEM. n.s., not significant, *P < 0.05. Statistical analyses were performed by one-way ANOVA