Fig. 6
From: Specific vulnerability of iPSC-derived motor neurons with TDP-43 gene mutation to oxidative stress
Evaluation of TDP-43 localization and TDP-43 RNA levels following oxidative stress. A Conditions of hydrogen peroxide treatment and timing of TDP-43 expression evaluation. B Immunostaining of TDP-43. Changes in TDP-43 localization were defined as positive when the cytoplasmic staining of TDP-43 was more intense than staining in the nucleus. Three experiments were performed, counting 50 cells per group per experiment. There was a trend toward an increase in the number of motor neurons in which TDP-43 localization was changed from the nucleus to the cytoplasm in the of A382T group. Scale bars, 20 μm. C RNA levels of TDP-43. TDP-43 RNA levels tended to increase in motor neurons in the A382T group when hydrogen peroxide was applied. B n = 5 in each of the three groups. The data are presented as the mean ± SEM. n.s., not significant, *P < 0.05. Statistical analyses were performed by one-way ANOVA. C n = 3 in each of the three groups. The data are presented as the mean ± SEM. n.s., not significant, *P < 0.05. Statistical analyses were performed by t test and one-way ANOVA