Fig. 1
From: Profiling tyrosine kinase inhibitors as AD therapeutics in a mouse model of AD
Comparison of the therapeutic effects of TKIs on AD pathology in 5xFAD mice. A Schematic illustration of amyloid pathology. B, C Immunofluorescence staining of 4G8 in brain slices from 5xFAD mice injected daily with vehicle (10% DMSO + 40% PEG300 + 5% Tween80 + 45% saline), ibrutinib (10 mg/kg, i.p.), PD180970 (10 mg/kg, i.p.), or cabozantinib (10 mg/kg, i.p.) for 14 consecutive days. D, E Quantification of data from B-C (n = 18–21 brain slices from 3–5 mice/group). F, G Immunofluorescence staining of NEP in brain slices from 5xFAD mice injected daily with vehicle, ibrutinib, PD180970, or cabozantinib for 14 consecutive days. H, I Quantification of data from F-G (n = 16–20 brain slices from 3–5 mice/group). J Schematic illustration of tau hyperphosphorylation. K–L Immunofluorescence staining of AT100 (K) or AT180 (L) in brain slices from 5xFAD mice injected daily with vehicle, ibrutinib, PD180970, or cabozantinib for 14 consecutive days. M Quantification of data from K (n = 19–20 brain slices from 4–5 mice/group). N Quantification of data from L (n = 15–20 brain slices from 4–5 mice/group). O, P Immunofluorescence staining of DYRK1A (O) or p-CDK5 (P) in brain slices from 5xFAD mice injected daily with vehicle, ibrutinib, PD180970, or cabozantinib for 14 consecutive days. Q Quantification of data from O (n = 19–20 brain slices from 4–5 mice/group). R Quantification of data from P (n = 19–20 brain slices from 4–5 mice/group). S Schematic illustration of neuroinflammation. T, V Immunofluorescence staining of Iba-1 in brain slices from 5xFAD mice injected daily with vehicle, ibrutinib, PD180970, or cabozantinib for 14 consecutive days. U, W Quantification of data from T and V, respectively (n = 20 brain slices from 4–5 mice/group). X Immunofluorescence staining of GFAP in brain slices from 5xFAD mice injected daily with vehicle, ibrutinib, PD180970, or cabozantinib for 14 consecutive days. Y Quantification of data from X (n = 20 brain slices from 4–5 mice/group). *p < 0.05, **p < 0.01, and ***p < 0.001. Scale bar = 100 μm