Fig. 1

A Diagram showing the experimental timeline for the retinal injection of either scAAV1.hSyn.GFP (n = 3) or scAAV1.short.CAG.GFP (n = 4). B Image of a retina injected with scAAV1.short.CAG.GFP. C, D Representative image showing GFP-labeled neurons in the SC after scAAV1.hSyn.GFP (C) and scAAV.1 short.CAG.GFP (D) retinal injection; arrows point to labelled neurons. E The number of GFP-positive neurons in the SC is significantly higher in mice with scAAV1.short.CAG.GFP retinal injection (n = 4) compared to mice with scAAV1.hSyn.GFP retinal injection (n = 3; Unpareid t-test, p = 0.0027). F–H Representative images from the ipsilateral SC (F), contralateral SC (G), and LGN (H). The insets below are high-magnification images of the boxed regions. I Diagram showing the injection of AAV.1 short.CAG.GFP in the MGn and the anterograde transsynaptically labeled neurons in the BLA (n = 4). J Representative image of the injection site in the MGn. K, L Representative images showing that the ipsilateral BLA has GFP-positive neurons; arrows point to labelled neurons (L) after AAV1.short.CAG.GFP in the MGn, while the contralateral BLA (K) does not. The boxes below are high magnification of the respective regions. Results are reported as mean ± SEM. **p < 0.01