Fig. 5
From: AAV-compatible optogenetic tools for activating endogenous calcium channels in vivo
Increasing Ca2+ in cultured astrocytes and microglia through activation of monSTIM1 variants. A Fluorescence images of RGECO1 in astrocytes (left) and microglia (BV2 cells, right) co-expressing each monSTIM1 variant, illuminated with blue light. B Schematic illustrating the protocol for light illumination. C Summary data showing relative changes in RGECO1 intensity over time upon light illumination. D Summary data showing maximum fold changes (ΔF/F0) in RGECO1 intensity upon activation of monSTIM1 variants. EGFP-monSTIM1: n = 16; FLAG-monSTIM1: n = 22; EGFP-CRY2-STIM1(318–450): n = 21; EGFP-IRES2-CRY2-STIM1(238–448): n = 21; EGFP-STIM1 + vhhGFP-CRY2: n = 18; CIBN-STIM1 + CRY2: n = 18 cells (astrocyte). EGFP-monSTIM1: n = 19; FLAG-monSTIM1: n = 31; EGFP-CRY2-STIM1(318–450): n = 11; EGFP-IRES2-CRY2-STIM1(238–448): n = 11; EGFP-STIM1 + vhhGFP-CRY2: n = 13; CIBN-STIM1 + CRY2: n = 18 cells (BV2). E Fluorescence images of RGECO1 in astrocytes upon light illumination of a subcellular region indicated by a white-lined circle. F Kymograph of RGECO1 corresponding to lines a-b and c-d in (E). Data are presented as means ± SEM (*p < 0.05, ****p < 0.0001; Student two-tailed t test); ns, not significant (p > 0.05)