Fig. 1

Characterization of brain biomechanics, histological features, and Piezo1 distribution pattern in diverse brain regions from the donors. (A) Coronal brain sections and representative H&E images demonstrating regional tissue architecture (scale bar = 100\(\mu\)m). (B) Quantification of viscoelastic properties across different brain locations. Donors are separated based on columns; Donor 1 (D1), Donor 2 (D2), Donor 3 (D3), and Donor 4 (D4); figure legend shows WM, Corona Radiata (CR), GW Junction from Right Parietal Lobe (RPL), GW Junction from Left Occipital Lobe (LOL), and Pons. One-way ANOVA demonstrates significant differences in spring term between the GW junction and white matter of the corona radiata in Donor 3. Similarly, the equilibrium stress of the GW junction was shown to be significantly different compared to the pons and white matter in donor 3. Only comparisons between the GW junction and pons present significant differences in equilibrium stress in donor 4, *p < 0.05, **p < 0.01. (C) Individualized comparison of Elastic Modulus (Pa) of different brain regions. (D) Representative immunofluorescent staining of brain regions showing Piezo1 (red) and cell nuclei (blue), scale bar = 20\(\mu\)m. Yellow arrows show examples of Piezo1 positive cells. (E) Quantification of the percentage of Piezo1 positive cells based on immunofluorescent (IF) staining presented in Fig. 1D; one-way ANOVA was used showing *p < 0.05, **p < 0.01, ****p < 0.0001. Created with BioRender.com