Fig. 2
R8p-specific knockdown of dnrx-1 disrupted the maintenance of R8y subtype identity by de-repressing Rh5 expression. (A-D and E-G) Frozen sections of adult heads expressing the Rh5 reporter Rh5 > nSyb-GFP, were stained with anti-GFP (green) and MAb24B10 (magenta). (A and A’) Wild type. (B and B’) Flies with R8p-specific expression of UAS-nrx-1-RNAi-GD2619 (n = 13). (C and C’) Flies with R8p-specific expression of UAS-nrx-1-RNAi-GD14451 (n = 14). (D) Significant increases in the percentage of Rh5-positive axons were observed with R8p-specific expression of two independent dnrx-1-RNAi transgenes UAS-nrx-1-RNAi-GD2619 and UAS-nrx-1-RNAi-GD14451. ***p < 0.001. Error bars indicate SD. Scale bar, 20 μm. (E and E’) GAL80ts control individuals (n = 13). (F and F’) Flies in which dnrx-1 was specifically knocked down at adult stage (n = 12). (G) Knockdown of dnrx-1 specifically at adult stage in R8p subtypes also significantly increased the percentage of Rh5-positive axons. ***p < 0.001. Error bars indicate SD. Scale bar, 20 μm. (H and I) Frozen sections of adult heads co-expressing Rh5 reporter Rh5 > syt-GFP and Rh6 reporter Rh6-lacZ were double-stained with anti-GFP (green) and anti-b-galactosidase (red). (H and H’) Wild type. (I and I’) In flies with R8p-specific expression of nrx-1-RNAi-GD14451, the Rh5 reporter was ectopically expressed in many R8y subtypes (arrows). Scale bar: 20 μm