Fig. 4

dnrx-1 knockdown did not show sex-based effects on the maintenance of Rh5 restriction. Frozen sections of adult heads expressing the Rh5 reporter Rh5 > Syt-GFP, were stained with anti-GFP (green) and MAb24B10 (magenta). (A-A’) Wild-type female flies (n = 8). (B-B’) Female flies with R8p-specific expression of nrx-1-GD14451 (n = 16). (C-C’) Wild-type male flies (n = 8). (D-D’) Male flies with R8p-specific expression of nrx-1-GD14451 (n = 16). (E) No significant difference in the percentage of Rh5-positive axons between female and male dnrx-1 knockdown flies was observed. ***p < 0.001. ns, p > 0.05. Error bars indicate SD. Scale bar: 20 μm