Fig. 5

Silencing R8p caused a dnrx-1-like phenotype. Frozen sections of adult heads expressing the Rh5 reporter Rh5 > Syt-GFP, were stained with anti-GFP (green) and MAb24B10 (magenta). (A-A’) Wild-type female flies (n = 5). (B-B’) Female flies in which Kir2.1 was specifically expressed in R8p subtypes (n = 5). (C) Silencing R8p with Kir2.1 significantly increased the percentage of Rh5-positive axons in both male and female flies. 5 male and 5 female flies in each genotype were examined. ***p < 0.001. ns, p > 0.05. Error bars indicate SD. Scale bar: 20 μm