Fig. 6

Knockdown of dnrx-1 did not affect the formation of active zones in R8 presynaptic terminals. Frozen sections of adult heads expressing Rh5/Rh6-brp-mCherry were stained with anti-dsRed (Red). (A) Wild type. (A’) Enlargement of the boxed area in (A) (B) dnrx-1 knockdown flies. (B’) Enlargement of the boxed area in (B). (C) The region of interest (ROI) was thresholded, and the percentage of Brp-positive area was quantified. While no decrease in the levels of Brp was observed in female dnrx-1 knockdown flies (n = 8), there was an increase in the levels of Brp in male dnrx-1 knockdown flies (n = 8). ***p < 0.05. ns, p > 0.05. Error bars indicate SD. Scale bar: 20 μm